An open µ-Slide (chambered coverslip) with 2 wells for use in immunofluorescence and high-end microscopy. Phase contrast microscopy is the most commonly used, transmitted light technique in cell culture. When working with phase contrast microscopy, it is crucial to have the two phase rings adjusted to each other. In open wells, the meniscus at the air-water-interphase works like a lens that refracts the beam path. This miscalibrates the phase rings, leading to poor contrast in the microscopic image.
Working with the µ-Slide 2 Well Ph+ diminishes the meniscus, so that the whole optical system is aligned, no matter which position of the well is being imaged.

• All-in-one chamber slide used for cell culture and microscopy
• Observation of the sample through the coverslip-like bottom using high resolution microscopy
• Fast and easy immunofluorescence, with no coverslips and no leakage
• Cost-effective experiments using small numbers of cells and low volumes of reagents
• Cultivation and microscopy of cell cultures
• Transfection assays
• Fluorescence microscopy of both living and fixed cells
• Live cell imaging over extended time periods
• Suitable for DIC, when used with a DIC lid